performa spin columns (Edge Biosystems Inc)

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Edge Biosystems Inc performa spin columns
Performa Spin Columns, supplied by Edge Biosystems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/performa+spin+columns/performa+spin+columns/pm38100082-62-7-10
Average 90 stars, based on 1 article reviews

performa spin columns - by Bioz Stars, 2026-10

90/100 stars

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Sequencing:

Article Title: A de novo 2.3 kb structural variant in MITF explains a novel splashed white phenotype in a Thoroughbred family.
Article Snippet: .. The sequencing product was cleaned up using Performa Spin Columns (Edge BioSystems) and visualized with a ABI 3730 Genetic Analyzer (Applied Biosystems, ThermoFisher Scientific). ..

Article Title: Phytophthora Species in Rivers and Streams of the Southwestern United States
Article Snippet: The remaining PCR product was incubated with ExoSap-It (Affymetrix, Santa Clara, CA) according to the manufacturer's instructions and sequenced with each amplification primer using the BigDye Terminator v3.1 cycle sequencing kit (Life Technologies, Grand Island, NY). .. Sequencing reaction products were cleaned on Performa spin columns (Edge Biosystems, Gaithersburg, MD) and loaded onto the ABI 3100 capillary sequencing platform (Life Technologies) at the New Mexico State University (NMSU) Molecular Core Facilities. .. Geneious v6.0.5 (Biomatters Ltd., Auckland, NZ) was used for all sequence analysis.

Article Title: Ipomoea gilana: A New and Endemic Morning Glory (Ipomoeeae, Convolvulaceae) in the Gila National Forest, New Mexico
Article Snippet: An endemic morning glory of southwestern New Mexico, Ipomoea gilana, known only from the Black Range of the Gila National Forest, is described and distinguished from a close phylogenetic associate, Ipomoea orizabensis (Ipomoea series Tyrianthinae), from mid-elevation mountains (800–2,000 m) of Mexico and Central America.. The new species resembles I. lindheimeri (Ipomoea section Pharbitis) superficially, but molecular studies indicate closer relationships with I. orizabensis.. Ipomoea gilana has slightly succulent leaf blades, nocturnal anthesis, light blue corollas, slightly exserted, subequal stamens with filaments inserted 2 cm above the base of the corolla tube and three-locular capsules.

Electrophoresis:

Article Title: The development of miniSTRs as a method for high-speed direct PCR.
Article Snippet: There are situations in which it would be very valuable to have a DNA profile within a short time; for example, in mass disasters or airport security.. In previous work, we have promoted reduced size STR amplicons for the analysis of degraded DNA.. We also noticed that shorter amplicons are more robust during amplification, making them inhibition resistant, and potentially applicable to high-speed direct PCR.

Polymerase Chain Reaction:

Purification:

Article Title: A Mechanism for microRNA Arm Switching Regulated by Uridylation.
Article Snippet: RNAs were transferred to Hybond-NX membranes (Amersham) and crosslinked to the membranes with EDC hydrochloride (Sigma-Aldrich) (Pall and Hamilton, 2008). .. Antisense probes were radiolabeled at their 50 ends with [g-32P] ATP by T4 polynucleotide kinase (Takara) and purified using Performa Spin Columns (Edge BioSystems). .. The membranes were incubated with denatured UltraPure Salmon Sperm DNA Solution (Thermo Scientific) in PerfectHyb Plus Hybridization Buffer, hybridized with antisense probes, and washed with mild wash buffer (0.05% SDS and 2X SSC) followed by stringent wash buffer (0.1% SDS and 0.1X SSC).

Article Title: MicroRNA arm switching regulated by uridylation
Article Snippet: RNAs were transferred to Hybond-NX membranes (Amersham) and crosslinked to the membranes with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide ( ). .. Antisense probes were radiolabeled at their 5′ends with [ γ - 32 P] ATP by T4 polynucleotide (Takara) and purified using Performa Spin Columns (Edge BioSystems). .. The membranes were incubated with denatured UltraPure Salmon Sperm DNA Solution (Thermo Scientific) in PerfectHyb Plus Hybridization Buffer, hybridized with antisense probes, and washed with mild wash buffer (0.05% SDS and 2X SSC) followed by stringent wash buffer (0.1% SDS and 0.1X SSC).

Centrifugation:

Article Title: Identification of Pol IV and RDR2-dependent precursors of 24 nt siRNAs guiding de novo DNA methylation in Arabidopsis
Article Snippet: 50 ml of washed NRPB2-FLAG or NRPD1-FLAG resin was mixed with 50 ml of 2 transcription reaction mix (18 nM M13mp18 template [Bayou Biolabs, Metairie, LA], 2 mM each rNTP, 120 mM ammonium sulfate, 40 mM HEPES-KOH pH 7.9, 24 mM magnesium sulfate, 20 uM zinc sulfate, 20 mM DTT, 20% glycerol, 1.6 U/ul RNase inhibitor) and incubated at room temperature for 1 hr on a rotating mixer. .. Reactions were subjected to centrifugation through Performa spin columns (EdgeBiosystems, Gaitherburg, MD), the filtrate was adjusted to 0.3M sodium acetate, and nucleic acids were precipitated with isopropanol. .. The pellets were resuspended in 8 ml water and DNase-treated using Turbo DNA-free kit reagent (ThermoFisher Scientific, Grand Island, NY).

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